【作者】 周帥; 薛俊杰; 劉艷芳; 楊焱; 張勁松; 馮娜; 羅璽; 唐慶九;
【Author】 ZHOU Shuai,XUE Junjie,LIU Yanfang,YANG Yan,ZHANG Jingsong,FENG Na,LUO Xi,TANG Qingjiu National Engineering Research Center of Edible Fungi;Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture;Shanghai Key Laboratory of Agricultural Genetics and Breeding;Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences,Shanghai 201403,China
【機構】 國家食用菌工程技術研究中心農業(yè)部應用真菌資源與利用重點開放實驗室上海市農業(yè)遺傳育種重點開放實驗室上海市農業(yè)科學院食用菌研究所;
【摘要】 采用高效陰離子色譜-脈沖安培檢測法(HAPEC-PAD)測定17種食用菌中海藻糖、甘露醇和阿糖醇含量。結果表明:阿糖醇、海藻糖和甘露醇的線性范圍分別為239.621.25、59.410.15、148.295.00μg/mL,檢出限分別為0.41、0.04、.11μg/mL,定量限分別為1.14、0.10和5.79μg/mL;17種食用菌中,香菇(Lentinula edodes)和猴頭菌(Hericiumerinaceus)中阿糖醇含量較高,茶樹菇(Agrocybe aegerita)、刺芹側耳(Pleurotus eryngii)、鮑魚側耳(Pleurotus abalonus)、松口蘑(Tricholoma matsutake)、蛹蟲草(Cordycepsmilitaris)中海藻糖含量較高,姬松茸(Agaricus blazei)、雞油菌(Cantharellus cibarius)、金頂側耳(Pleurotus citrinopileatus)、冬蟲夏草(Cordyceps sinensis)中甘露醇含量較高;該...
【Abstract】 A method for determining trehalose,mannitol and arabitol levels in the fruit bodies of 17 edible fungi using high performance anion chromatography-pulsed amperometric detection(HAPEC-PAD) is described.Samples could be tested without derivatization,and arabitol,trehalose and mannitol measurements were linear over the ranges 239.62-1.25,59.41-0.15 and 148.29-5.00 μg/mL,respectively.The limits of detection(LOD) for arabitol,trehalose and mannitol were 0.41,0.04 and 2.11μg/mL,respectively and the li...
【關鍵詞】 食用菌; 高效陰離子色譜; 脈沖安培檢測器; 海藻糖; 甘露醇; 阿糖醇;
【Key words】 Edible fungi; high performance anion chromatography; pulsed amperometric detection; trehalose; mannitol; arabitol;
【基金】 上海市科技興農重點攻關項目[編號:滬農科攻字(2009)第2-2號]的部分研究內容
【分類號】S646