【作者】 姚強(qiáng); 高興喜; 宮志遠(yuǎn); 孔明; 任鵬飛; 劉巖; 韓建東; 任海霞;
【Author】 YAO Qiang1,GAO Xingxi2,GONG Zhiyuan1,KONG Ming2,REN Pengfei1,LIU Yan3,HAN Jiandong1,REN Haixia1 1Soil and Fertilizer Institute,Shandong Academy of Agricultural Sciences,Jinan,Shandong 250100,China;2Institute of Mycological Science and Technology,Ludong University,Yantai,Shandong 264025,China;3School of Life Sciences,Qingdao Agricultural University,Qingdao,Shandong 266109,China
【機(jī)構(gòu)】 山東省農(nóng)業(yè)科學(xué)院土壤肥料研究所; 魯東大學(xué)菌物科學(xué)與技術(shù)研究院; 青島農(nóng)業(yè)大學(xué)生命科學(xué)學(xué)院;
【摘要】 研究硬脂酸、亞油酸和棕櫚酸對(duì)靈芝液體發(fā)酵的影響。結(jié)果表明,3種供試脂肪酸都能促進(jìn)靈芝菌絲體生物量的積累;硬脂酸有利于靈芝胞外多糖的產(chǎn)生,在添加濃度為1.0g/L時(shí),胞外多糖量達(dá)到(1 066.74±11.27)mg/L,是對(duì)照的3.37倍;亞油酸利于胞內(nèi)多糖的合成,在濃度為1.5g/L時(shí),胞內(nèi)多糖量達(dá)到(620.90±7.11)mg/L,是對(duì)照的2.56倍;另外,亞油酸還對(duì)靈芝胞內(nèi)三萜和胞外三萜量表現(xiàn)出較強(qiáng)的促進(jìn)作用,在濃度為2.0 g/L時(shí),胞內(nèi)及胞外三萜量分別達(dá)到(394.23±10.32)mg/L和(551.32±6.22)mg/L,分別比對(duì)照提高了1.13和1.12倍;而硬脂酸和棕櫚酸的添加則降低胞內(nèi)和胞外三萜的量。
【Abstract】 The effects of supplementing Ganoderma lucidum growth medium with different concentrations of stearic,linoleic and palmitic acids were assessed in terms of mycelial biomass,extracellular and intracellular polysaccharide yields,and intracellular and extracellular triterpene levels.Mycelial biomass increased with increasing concentrations of all three fatty acids over the range 0-2.0 g/L.Highest yields of extracellular and intracellular polysaccharide were recorded using media supplemented with 1....
【關(guān)鍵詞】 靈芝; 硬脂酸; 亞油酸; 棕櫚酸; 多糖; 三萜類物質(zhì);
【Key words】 Ganoderma lucidum; stearic acid; linoleic acid; palmitic acid; polysaccharide; triterpenoids;
【基金】 山東省自然科學(xué)基金項(xiàng)目(編號(hào):Q2008D02);;國(guó)家科技支撐計(jì)劃項(xiàng)目(編號(hào):2007BAD89B09-11);;山東省農(nóng)業(yè)良種工程項(xiàng)目(編號(hào):2007LZ06-3);;“泰山學(xué)者”建設(shè)工程專項(xiàng)經(jīng)費(fèi)項(xiàng)目;;魯東大學(xué)食(藥)用真菌創(chuàng)新團(tuán)隊(duì)項(xiàng)目的部分研究?jī)?nèi)容
【分類號(hào)】S567.31