【作者】劉建忠; 孫淑靜; 胡開輝; 熊芳; 肖雅敏; 陳明祥; 饒榆平; 張俊蘭

【Author】LIU Jian-zhong; SUN Shu-jing; HU Kai-hui; XIONG Fang; XIAO Ya-min; CHEN Ming-xiang; RAO Yu-ping; ZHANG Jun-lan(College of Life Sciences; Fujian Agriculture and Forestry University; Fuzhou Fujian 350002)

【作者機(jī)構(gòu)】福建農(nóng)林大學(xué)生命科學(xué)學(xué)院

【摘要】 運(yùn)用原生質(zhì)體融合與漆酶轉(zhuǎn)化體系篩選到一株真姬菇融合菌株(金山1號(hào)),研究了不同培養(yǎng)基配方、溫度、pH及含水量對(duì)該菌株菌絲生長(zhǎng)的影響,并對(duì)該菌株的漆酶活性、生產(chǎn)特性進(jìn)行了初步分析。結(jié)果表明,金山1號(hào)在PDA胡蘿卜培養(yǎng)基上菌絲生長(zhǎng)最快、活力較強(qiáng),菌絲最適生長(zhǎng)溫度為25℃,最適pH為6.5,最適含水量60%～65%,漆酶活力為3.95 U·mL-1,生產(chǎn)周期可縮短3 d～5 d,生物學(xué)效率72.86%,與真姬菇相比提高了17.15%,表現(xiàn)出該融合菌株具有良好的生產(chǎn)應(yīng)用價(jià)值。

【Abstract】 A Hypsizigus marmoreus fusion strain(Jinshan No.1) was screened by the technology of protoplast fusion and laccase transformation system.The effects of different culture media,temperature,pH and water content on the mycelial growth of fusion strain of Hypsizigus marmoreus were studied,and laccase activity and production characteristics of the fusion strain were preliminarily analysed.The experimental results showed that Jinshan No.1 strain had highest mycelial growth rate and the strongest mycelial growth vigor on the PDA carrot medium,and the optimum temperature,pH and water content were 25℃,pH 6.5,60%～65%.The laccase activity was 3.95 U·mL-1,and the biological efficiency was 72.86%,which increased 17.15% comparing with Hypsizigus marmoreus.The fruiting test results indicated that the fusion strain(Jinshan No.1) has good production value.

【關(guān)鍵詞】 真姬菇; 生物學(xué)特性; 融合子

【Key words】 Hypsizigus marmoreus; Biological characteristics; Fusion strain

【基金】福建省發(fā)改委農(nóng)業(yè)“五新”推廣項(xiàng)目,編號(hào):(2008)1119;; 福建省自然基金(2009J05046);; 福建省科技重點(diǎn)項(xiàng)目(2009N0010);; 福建省教育廳項(xiàng)目(JA09098);; 福建省教育廳項(xiàng)目(JA09097)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2010年03期

【DOI】CNKI:SUN:ZSYJ.0.2010-03-012

【分類號(hào)】S646.9