【作者】李洪榮; 陳美元; 廖劍華; 王澤生
【Author】LI Hongrong; CHEN Meiyuan; LIAO Jianhua; WANG ZeshengEdible Fungi Institute of Fujian Academy of Agricultural Sciences; Fuzhou; Fujian 350014; China
【作者機(jī)構(gòu)】福建省農(nóng)業(yè)科學(xué)院食用菌研究所
【摘要】 采用同工酶聚丙烯酰胺凝膠電泳(polyacrylamide gelelectrophoresis,PAGE)技術(shù),對(duì)雙孢蘑菇(Agaricus bisporus)菌株0214及其分解基質(zhì)能力退化菌株0214-3、0214-5在4種不同液體培養(yǎng)基中培養(yǎng)的菌絲體的酯酶(esterase,EST)、多酚氧化酶(polyphenoloxidase,PPO)、細(xì)胞色素氧化酶(cytochrome oxidase,COD)同工酶進(jìn)行分析,結(jié)果表明:在部分培養(yǎng)基中3個(gè)菌株的EST和PPO同工酶表型有明顯差異,且2個(gè)退化菌株的同工酶表型變異一致,說(shuō)明雙孢蘑菇分解基質(zhì)能力的退化與EST和PPO同工酶活性存在相關(guān)性。各菌株在不同培養(yǎng)基中的COD同工酶酶譜表型一致。
【Abstract】 Esterase(EST),polyphenoloxidase(PPO) and cytochrome oxidase(COD) isozyme patterns were determined in Agaricus bisporus,strain 0214,and two derived mutant strains(0214-3 and 0214-5) with diminished ability to degrade the cultivation substrate,following growth on four different liquid media.Although the EST and PPO isozyme patterns of the two mutants were virtually identical under every growth condition,they differed from the EST and PPO isozyme patterns of strain 0214 observed following growth on two and three test media,respectively.All three strains exhibited the same COD isozyme patterns irrespective of the growth conditions.Our data indicated a link between EST and PPO isozyme patterns and the substrate-decomposing ability of A.bisporus.
【關(guān)鍵詞】 雙孢蘑菇; 聚丙烯酰胺凝膠電泳; 退化; 同工酶
【Key words】 Agaricus bisporus; cultivation substrate-degrading ability; isozyme; esterase; polyphenoloxidase
【基金】國(guó)家自然科學(xué)基金項(xiàng)目(編號(hào):30871769);; 福建省科技重大專題(編號(hào):2008SZ0002)的部分研究?jī)?nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年02期
【DOI】CNKI:SUN:SYJB.0.2010-02-010
【分類號(hào)】S646.11