【作者】鮑大鵬; 趙國屏; 譚琦; 王升躍; 陳明杰; 鄭華軍; 張勁松; 朱永強; 汪虹; 康慧; 陳祥; 林楠; 馮愛萍

【Author】BAO Dapeng1; ZHAO Guoping2; TAN Qi1; WANG Shengyue2; CHEN Mingjie1; ZHENG Huajun2; ZHANG Jinsong1; ZHU Yongqiang2; WANG Hong1; KANG Hui2; CHEN Xiang2; LIN Nan1; FENG Aiping11National Engineering Research Center of Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; People’s Republic of China; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China 2Chinese National Human Genome Center at Shanghai; Shanghai 201203; China

【作者機構(gòu)】國家食用菌工程技術(shù)研究中心; 農(nóng)業(yè)部應用真菌資源與利用重點開放實驗室; 上海市農(nóng)業(yè)遺傳育種重點開放實驗室; 上海市農(nóng)業(yè)科學院食用菌研究所; 國家人類基因組南方研究中心

【摘要】 采用Roche 454 GS FLX測序技術(shù)和Solexa測序技術(shù)相結(jié)合,進行了草菇(Volvariella volvacea)全基因組的測序,最終獲得了長度為35.67Mb的草菇全基因組序列。通過生物信息學預測,獲得了11097個蛋白編碼基因,其中5516個基因有明確的功能注釋信息。這是在國際上首次報道的草菇基因組框架圖。

【Abstract】 The two next-generation high-throughput approaches of Roche 454 GS FLX and Illumina Solexa have been used to sequence the genome of Volvariella volvacea. The genome is 35.67 Mb in size and encodes 11 097 proteins based on the bioinformatics prediction. Among the total predicted genes,5 516 genes have been assigned either a definitive or tentative function. This represents the first report of a draft V. volvacea genomic sequence.

【關(guān)鍵詞】 草菇; 全基因組; 框架圖

【Key words】 Volvariella volvacea; genome; draft sequence

【基金】上海市科委科技攻關(guān)重點項目“草菇基因組和功能基因組數(shù)據(jù)庫共享平臺建設(shè)”(編號:10DZ2212400)的部分研究內(nèi)容;; 上海市科學技術(shù)委員會的資助,資助課題編號為10DZ2212400~~

【文獻出處】 食用菌學報,Acta Edulis Fungi,編輯部郵箱,2010年01期

【DOI】CNKI:SUN:SYJB.0.2010-01-001

【分類號】S646.13