【作者】孫曉紅; 陳明杰; 汪虹; 馮愛萍; 潘迎捷

【Author】SUN Xiaohong1; 2; 3; CHEN Mingjie1; WANG Hong1; FENG Aiping1; PAN Yingjie31National Engineering Research Center of Edible Fungi; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; Shanghai Key Laboratory of Agricultural Genetics and Breeding; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; 201106; P. R. China; 2College of Life Sciences; Nanjing Agricultural University; Nanjing; Jiangsu 210095; China; 3Shanghai Ocean University; Shanghai 200090; China

【作者機(jī)構(gòu)】國(guó)家食用菌工程技術(shù)研究中心; 農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開放實(shí)驗(yàn)室; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開放實(shí)驗(yàn)室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 南京農(nóng)業(yè)大學(xué)生命技術(shù)學(xué)院; 上海海洋大學(xué)

【摘要】 根據(jù)草菇(Volvariella volvacea)S-腺苷-L-高半胱氨酸水解酶基因(SAHH)全長(zhǎng)cDNA序列設(shè)計(jì)引物,以草菇基因組DNA為模板,PCR擴(kuò)增獲得草菇SAHH基因的全長(zhǎng)序列,序列分析結(jié)果表明,草菇SAHH長(zhǎng)度為1868bp,編碼430個(gè)氨基酸殘基,基因由12個(gè)外顯子和11個(gè)內(nèi)含子組成,將草菇SAHH基因推斷的氨基酸序列與其它物種的SAHH基因氨基酸序列進(jìn)行比較發(fā)現(xiàn),SAHH基因保守性較高。

【Abstract】 The genomic sequence of a S-adenosyl-L-homocysteine hydrolase gene (SAHH) from Volvariella volvacea was amplified using specific primers designed according to the full-length SAHH cDNA. SAHH consists of 1 868 bp encoding 430 amino acids,and contains 12 exons and 11 introns. A phylogenetic tree constructed on the basis of the amino acid sequences of SAHH genes in V. volvacea and other reference species revealed a high degree of conservation.

【關(guān)鍵詞】 草菇; S-腺苷-L-高半胱氨酸水解酶基因; 克隆; 序列分析

【Key words】 Volvariella volvacea; S-adenosyl-L-homocysteine hydrolase gene; clone; sequence analysis

【基金】農(nóng)業(yè)部公益性行業(yè)科研專項(xiàng)項(xiàng)目(編號(hào):nyhyzx07-008);; 上海市科技興農(nóng)重點(diǎn)攻關(guān)項(xiàng)目[編號(hào):滬農(nóng)科攻字(2007)第6-2號(hào)]的部分研究?jī)?nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年01期

【DOI】CNKI:SUN:SYJB.0.2010-01-006

【分類號(hào)】S646.13