【作者】賈薇; 嚴(yán)淑玲; 樊華

【Author】JIA Wei1; 2; YAN Shuling2; FAN Hua21National Research Center for Edible Fungi Biotechnology and Engineering; Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; People’s Republic of China; Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Research Center of Natural Medicinal Resources; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; 2Institut fr Molekularbiologie und Biochemie; Charite Universit|t –Medicine Berlin; Arnimallee 22; D-14195 Berlin; Germany

【作者機(jī)構(gòu)】國(guó)家食用菌工程技術(shù)研究中心; 農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開放實(shí)驗(yàn)室; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開放實(shí)驗(yàn)室; 上海市農(nóng)業(yè)科學(xué)院天然藥用資源研究與開發(fā)中心; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所; 柏林夏洛蒂醫(yī)科大學(xué)分子生物學(xué)和生物化學(xué)研究所

【摘要】 姬松茸(Agaricus blazei)子實(shí)體經(jīng)沸水提取、乙醇沉淀、DEAE-Sepharose Fast Flow和Sephacryl S-300HR層析獲得多糖ABMB3,研究表明:200μg/mL ABMB3可以刺激小鼠淋巴細(xì)胞分泌免疫球蛋白G(Immunoglobulin G,IgG)、IgM以及細(xì)胞因子白細(xì)胞介素6(Interleukin6,IL-6)、干擾素(Interferon,IFN)、IL-2和IL-4,說明ABMB3可以提高小鼠的免疫功能。

【Abstract】 A polysaccharide fraction,ABMB3,was obtained from fruit bodies of Agaricus blazei following extraction with boiling water,ethanol precipitation ,ion exchange chromatography (DEAE-Sepharose Fast Flow) and gel filtration chromatography (Sephacryl S-300HR). ABMB3 at a concentration of 200 μg/mL improved murine immunological functions by enhancing levels of IgG,IgM,IL-6,IFNg,IL-2 and IL-4.

【關(guān)鍵詞】 姬松茸; 多糖; 免疫活性

【Key words】 Agaricus blazei; polysaccharide; immunological function

【基金】德國(guó)Bundes ministriumfr Forschungund Technologie, Bonn科研基金資助項(xiàng)目(編號(hào):SFB366)的部分研究?jī)?nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2010年01期

【DOI】CNKI:SUN:SYJB.0.2010-01-015

【分類號(hào)】S567.39