沈?qū)W香; 尚曉冬; 汪虹; 譚琦
農(nóng)業(yè)部應(yīng)用真菌資源與利用重點(diǎn)開放實(shí)驗(yàn)室; 上海市食用菌工程技術(shù)研究中心; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室; 上海市農(nóng)業(yè)科學(xué)院食用菌研究所
【中文摘要】 用對(duì)峙培養(yǎng)技術(shù)在PDA平板上對(duì)10株香菇菌株的營養(yǎng)體不親合性進(jìn)行了研究。結(jié)果表明,供試菌株的營養(yǎng)體不親合性表現(xiàn)可分為兩種不親合類型和一種親合類型。在2次重復(fù)實(shí)驗(yàn)中表現(xiàn)完全一致的有48對(duì)組合,占87.3%;表現(xiàn)不同但仍可認(rèn)為是親合類型而不影響結(jié)果性質(zhì)的有4例,占7.3%,分別是135×66、9015×66、9015×7402和Cr04×野生50;表現(xiàn)出相反的結(jié)果,影響親合性判定的有3例占5.4%,分別是Cr04×26、Cr04×申香10號(hào)和26×蘇香。11株供試香菇菌株間營養(yǎng)體不親合性的表現(xiàn)以不親合類型為主,第一種和第二種不親合類型分別出現(xiàn)86次(78.2%)和13次(11.8%);親合類型(第三種類型)較少,有11次(10%)。135、939和9015間彼此親合,它們與其它菌株均表現(xiàn)不親合。申香10號(hào)與26親合,2菌株與除Cr04和蘇香以外的菌株均不親合。
【英文摘要】 Somatic incompatibility (SI) between ten cultivars and one wild-type strain of Lentinula edodes was determined from mycelial growth confrontation reactions on potato dextrose agar plates. Two types of SI reactions were observed, together with one type of somatic compatibility (SC) reaction. Identical SI or SC reactions were recorded in duplicate confrontation tests between 48 pairs of L. edodes strains yielded, accounting for 87.3% of the total 110 test pairings. Different SI reactions were observed in duplicate pairings between strains 135 and 66, 9015 and 66, 9015 and 7402, and Cr04 and 50 (7.3% of the total). The opposite reaction (from SI to SC or vice versa) was observed in three sets of duplicate confrontation tests (5.4% of the total pairings) involving strains Cr04 and 26, Cr04 and Shenxiang 10, and 26 and Suxiang. The two SI reaction types were the main responses between the eleven test strains and accounted for 90% of the total. Strains 9015, 135 and 939 were somatically com...更多patible with each other but somatic incompatible with the remaining strains. Strains Shenxiang 10 and 26 were somatically compatible with each other but somatically incompatible with all the other strains except Cr04 and Suxiang. 還原
【中文關(guān)鍵詞】 香菇; 營養(yǎng)體不親合性; 拮抗反應(yīng)
【英文關(guān)鍵詞】 Lentinula edodes; somatic incompatibility; antagonism reaetion
【基金】上海市農(nóng)委科技興農(nóng)重點(diǎn)攻關(guān)“香菇純‘自交系’育種技術(shù)的應(yīng)用研究”的部分研究內(nèi)容
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年02期 【DOI】CNKI:SUN:SYJB.0.2009-02-001