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  • Optimization of Conditions for Extracting Intracellular Polysaccharide with Anti-tumor Activity from


    【發(fā)布日期】:2010-05-17

    LU Zhaoshuang; WANG Guangyuan; ZHAO Chen; SONG Airong; MA HongsuoThe Institute of Medical Fungi Qingdao Agricultural University; Laboratory of Applied Mycologyof Shandong Province; Qingdao; Shandong 266109; China

    【中文摘要】 The Box-Behnken design was used to determine the optimal time,temperature and solvent∶solid ratio for extracting intracellular polysaccharide from G.frondosa mycelium produced under submerged culture conditions.Application of response surface methodology predicted a maximum polysaccharide extraction yield of 7.28% using an extraction time of 2.24 h,an extraction temperature of 92.53 ℃,and a water∶mycelium ratio of 35.76∶1.Under these conditions,the actual yield was 7.15%.Both low(200 mg/kg/d)and...更多 high(500 mg/kg/d)doses of G.frondosa polysaccharide,and positive controls administered 20 mg/kg/d cyclophosphamide,significantly inhibited the growth of S180 tumors with inhibition values of 61.5%,44.42% and 73.8%,respectively compared with negative controls administered physiological saline.  還原

    【英文摘要】 The Box-Behnken design was used to determine the optimal time,temperature and solvent∶solid ratio for extracting intracellular polysaccharide from G.frondosa mycelium produced under submerged culture conditions.Application of response surface methodology predicted a maximum polysaccharide extraction yield of 7.28% using an extraction time of 2.24 h,an extraction temperature of 92.53 ℃,and a water∶mycelium ratio of 35.76∶1.Under these conditions,the actual yield was 7.15%.Both low(200 mg/kg/d)and high(500 mg/kg/d)doses of G.frondosa polysaccharide,and positive controls administered 20 mg/kg/d cyclophosphamide,significantly inhibited the growth of S180 tumors with inhibition values of 61.5%,44.42% and 73.8%,respectively compared with negative controls administered physiological saline.

    【英文關(guān)鍵詞】 Grifola frondosa; intracellular polysaccharide; Box-Behnken design; S180 tumor
    【基金】Sponsored by the Government of Shandong Province(No.2007LZ07)

    【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2009年01期 【DOI】CNKI:SUN:SYJB.0.2009-01-014

     
     
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