ZHANG Meiyan~1 SHANG Xiaodong~1 TAN Qi~(1*) CHEN Mingjie~1 PAN Yingjie~2(~1Key Laboratory of Applied Mycological Resources and Utilization; Ministry of Agriculture; the People’s Republic of China; Shanghai Research Center for Edible Fungi Biotechnology and Engineering; Key Laboratory of Agricultural Genetics and Breeding of Shanghai; Institute of Edible Fungi; Shanghai Academy of Agricultural Sciences; Shanghai 201106; China; ~2Shanghai Fisheries University; Shanghai 200090; P.R.China)
【中文摘要】 <正>PCR amplification employing a SCAR (Sequence Characterized Amplified Region) primer pair, 135F/R,(amplifying a band of 601 bp) for identifying '135' strains of Lentinula dodes was used to establish the distribution of the specific SCAR marker among 58 protoplast monokaryons and 97 spore monokaryons isolated from a L.edodes 135 strain.Protoplast monok.aryons segregated into either A1B1 or A2B2 mating type,and the SCAR marker was detected only in the latter.The marker was also detected in...更多 four delineated mating types (A1B1,A2B2,A1B2,A2B1) of spore monokaryons.Our data testify to the stable inheritance of the specific SCAR marker,and show that the SCAR locus is not genetically linked to either the A or B mating factors. 還原
【英文摘要】 PCR amplification employing a SCAR (Sequence Characterized Amplified Region) primer pair, 135F/R,(amplifying a band of 601 bp) for identifying '135' strains of Lentinula dodes was used to establish the distribution of the specific SCAR marker among 58 protoplast monokaryons and 97 spore monokaryons isolated from a L.edodes 135 strain.Protoplast monok.aryons segregated into either A1B1 or A2B2 mating type,and the SCAR marker was detected only in the latter.The marker was also detected in four delineated mating types (A1B1,A2B2,A1B2,A2B1) of spore monokaryons.Our data testify to the stable inheritance of the specific SCAR marker,and show that the SCAR locus is not genetically linked to either the A or B mating factors.
【英文關(guān)鍵詞】 Inheritance patterns; Lentinula edodes; mating type; strain-specific sequence characterized amplified region (SCAR) marker
【基金】Supported by the Foundation of Shanghai Academy of Agricultural Sciences (No.2004-2-16)
【文獻(xiàn)出處】 食用菌學(xué)報,Acta Edulis Fungi,編輯部郵箱,2008年04期 【DOI】CNKI:SUN:SYJB.0.2008-04-004