黃春燕; 單洪濤; 劉傳富; 皇傳華; 萬(wàn)魯長(zhǎng); 郭惠東; 楊化恩; 張福進(jìn)
山東省農(nóng)科院植保所; 山東省農(nóng)科院植保所; 濟(jì)南市農(nóng)科所; 濟(jì)南市農(nóng)科所; 山東省農(nóng)科院植保所; 山東省農(nóng)科院植保所; 臨沂市河?xùn)|區(qū)農(nóng)業(yè)局; 威海市環(huán)翠區(qū)植保站 濟(jì)南250100 ; 濟(jì)南250100 ; 濟(jì)南250023 ; 濟(jì)南250023 ; 濟(jì)南250100 ; 濟(jì)南250100 ; 臨沂276000 ; 威海264000

【中文摘要】 研究了糙皮側(cè)耳液體菌種適宜的培養(yǎng)條件、培養(yǎng)周期與貯存期。結(jié)果表明,糙皮側(cè)耳液體菌種適宜的培養(yǎng)基配方為蔗糖2%,玉米粉4%,麥麩3%,酵母粉0.3%,KH2PO40.25%,Mg SO4·7H2O0.1%,ZnSO40.05%,pH6.17。搖瓶培養(yǎng)裝液量80mL/250mL,搖床轉(zhuǎn)速150r/min,接種量10%,培養(yǎng)周期6d,菌種于3d內(nèi)用完為宜。

【英文摘要】 The suitable culture conditions, the culture period and the preservation time of the liquid spawn of P. ostreatus were studied. The experiment results showed that the optimum formula for P. ostreatus liquid spawn medium was as follows: sucrose 2%, corn meal 4%, wheat bran 3%, yeast powder 0.3%, KH_2PO_4 0.25%, MgSO_4·7H_2O 0.1%, ZnSO_4 0.05%, and the optimum pH was 6.17. While the optimum medium capacity, the rotation rate, the spawning rate and the culture period were 80mL/250mL, 150r/min,10% and 6d resp.. And it would be better to use the spawn within 3 days.

【中文關(guān)鍵詞】 糙皮側(cè)耳; 液體菌種; 培養(yǎng)條件; 培養(yǎng)周期; 貯存期
【英文關(guān)鍵詞】 Pleurotus ostreatus; Liquid spawn; Culture condition; Culture period; Preservation time
【基金】山東省濟(jì)南市科技局科技攻關(guān)項(xiàng)目"食用菌菌種引進(jìn)選育及研究"[濟(jì)科合字(2001)第70-1號(hào)]的部分內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年04期 【DOI】CNKI:SUN:SYJB.0.2004-04-009