劉艷如; 余萍; 鄭怡
福建師范大學(xué)生物工程學(xué)院; 福建師范大學(xué)化學(xué)與材料學(xué)院; 福建師范大學(xué)生物工程學(xué)院 福州350007 ; 福州350007 ; 福州350007

【中文摘要】 經(jīng)硫酸銨分級(jí)沉淀、活性炭柱吸附、DEAE SepharoseFF離子交換層析及SephadexG 100分子篩層析,從柱狀田頭菇子實(shí)體浸出液中分離到在PAGE上顯示1條蛋白質(zhì)染色帶的凝集素ACL(Agrocybecylindracealectin)。經(jīng)SephadexG 100測(cè)得ACL分子量為67890,經(jīng)SDS PAGE測(cè)定,其亞基分子量分別為34673、20000和13183。ACL的中性糖含量為5.87%,氨基酸中天冬氨酸含量最高,半胱氨酸含量最低。ACL能凝集多種動(dòng)物和人的A、B或O型紅細(xì)胞,對(duì)鼠紅細(xì)胞的血凝活性可被7種單糖和3種雙糖抑制。ACL是一種對(duì)熱較為穩(wěn)定的凝集素。

【英文摘要】 Agrocybe cylindracea lectin (ACL) was isolated and purified from Agrocybe cylindracea sporophore by extraction with PBS followed by (NH_4)_2SO_4 fractionation precipitation, absorption in active carbon column, ion-exchange chromatography on DEAE-Sepharose FF and gel filtration on Sephadex G-100. The purified lectin showed a single protein band in PAGE and an molecular weight of about 67890 was measured when sephadex G-100 was used. The ACL, showing three protein bands in SDS-PAGE was composed of three kinds of subunits which had a molecular weight of 34673, 20000 and 13183 respectively. And it was proved in our determination that the ACL containing 5.87% neutral saccharide could hem agglutinate the erythrocytes of many kinds of animal blood and human blood with A, B and O blood types and its hemagglutinative activity to the mouse erythrocyte was inhibited by monosaccharide and disaccharide tested, and that the ACL was insensitive to temperature change under 50℃, while in its amino acid...更多 composition, the content of Asp was the highest and the content of Cyswas the lowest.  還原

【中文關(guān)鍵詞】 柱狀田頭菇; 凝集素; 提純; 血球凝集活性; 理化特性
【英文關(guān)鍵詞】 Agrocybe cylindracea; Lectin; Purification; Hemagglutination activity; Characteristic
【基金】福建省教育廳資助項(xiàng)目"食用菌凝集素的研究及應(yīng)用"(JA02185)的部分內(nèi)容

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,2004年03期 【DOI】CNKI:SUN:SYJB.0.2004-03-004