王南; 鮑大鵬; 黃禮濱; 譚琦; 潘迎捷
農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室; 農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106 ; 上海市農(nóng)業(yè)遺傳育種重點(diǎn)開(kāi)放實(shí)驗(yàn)室 上海市農(nóng)業(yè)科學(xué)院食用菌研究所 上海 201106
【中文摘要】 以不同來(lái)源的柱狀田頭菇(Agrocybe aegerita)為研究對(duì)象,就不同的碳氮比、pH值以及培養(yǎng)溫度對(duì)菌絲生長(zhǎng)速度和結(jié)實(shí)性的影響進(jìn)行了比較研究,并從同工酶角度分析比較了各菌株的親緣關(guān)系。研究結(jié)果表明,不同來(lái)源的柱狀田頭菇所要求的最佳培養(yǎng)條件不盡相同。一般情況下,菌絲在碳氮比為10:1～80:1時(shí)均可生長(zhǎng),碳氮比為10:1時(shí),菌絲生長(zhǎng)最快,且易結(jié)實(shí);柱狀田頭菇可生長(zhǎng)并結(jié)實(shí)的pH值為4～12,最適宜結(jié)實(shí)的pH值為6;25℃時(shí)柱狀田頭菇菌絲生長(zhǎng)速度最快,而20～25℃比較適合其結(jié)實(shí)。同工酶分析表明,柱狀田頭菇菌絲中過(guò)氧化物同工酶活性不高,酶帶單調(diào),不適宜用做檢測(cè)遺傳多樣性的生化標(biāo)記;酯酶同工酶有一定的活性,可以從相似系數(shù)上分析不同菌株的親緣關(guān)系。
【英文摘要】 Agrocybe aegerita strains from different countries and areas were collected. The effect of carbon nitrogen ratio, pH and temperature on mycelial growth and fecundity of A. aegerita. strains was compared. Meanwhile the isozyme tests were applied to analyze the relationship of various strains. The results indicated that the optimal culture conditions were not all the same for A. aegerita strains selected from different sources. Normally, the C/N and pH range suitable for mycelial growth and fruitbody formation were 10:1 - 80:1 and pH4 - 12 resp. , while the optimum C/N and temperature for mycelial growth were 10:1 and 25℃ resp. and optimum pH and temperature for fruitbody formation were pH 6 and 20-25℃ resp. . According to the results of isozyme tests, the per-oxidase isozyme activity of the mycelia was low and the isozyme bands were dull. So, peroxidase isozyme could not be used as biochemical mark for examining genetic diversity. Neverthless, the esterase isozyme activity of mycelia wa...更多s higher and the isozyme bands were more abundant, so it could be applied to analyze the relationship of different A. aegerita strains. 還原
【中文關(guān)鍵詞】 柱狀田頭菇; 培養(yǎng)條件; 同工酶
【英文關(guān)鍵詞】 Agrocybe aegerita; Culture condition; Isozyme
【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1999年03期 【DOI】CNKI:SUN:SYJB.0.1999-03-000