何強(qiáng)泰; 陸佩洪; 余多慰
南京師范大學(xué)生物系細(xì)胞遺傳教研室!南京210024; 南京師范大學(xué)生物系細(xì)胞遺傳教研室!南京210024; 南京師范大學(xué)生物系細(xì)胞遺傳教研室!南京210024

【中文摘要】 糙皮側(cè)耳菌絲體在含2.5％溶壁酶的0.6M KC1高滲穩(wěn)定液中,25℃說(shuō)解48～57小時(shí),或在37℃酶解4小時(shí)后再在25℃酶解47小時(shí),皆可產(chǎn)生直徑36.84μm左右的巨原生質(zhì)體,較一般正常原生質(zhì)體增大7～10倍左右。觀(guān)察巨原生質(zhì)體超微結(jié)構(gòu),發(fā)現(xiàn)其中液泡體積相應(yīng)增加、線(xiàn)粒體和內(nèi)容物增多、細(xì)胞核數(shù)目沒(méi)有變化,并觀(guān)察到巨原生質(zhì)體的再生。

【英文摘要】 When mycelia of Pleurotus ostreatus were incubated in 0.6M KC1 hypertonic solution with 2.5% lywallzyme at 25℃ for 48-57 hr.; or after enzymolysis at 37℃ for 4 hr. and incubation at 25℃ for 47 hr., the giant protoplast with diameter of 36.84um was obtained. Its average diameter could be 7-10 times greater than that of normal protoplast. Ultrastructure of the giant protoplast was studied with electron microscope. The results showed that the vacuole volume became large, the contents and the mitochondrion increased, but the numbers of nuclei were unchanged. And the regeneration of giant protoplast was also observed.

【中文關(guān)鍵詞】 食用菌; 巨原生質(zhì)體; 超微結(jié)構(gòu)
【英文關(guān)鍵詞】 Edible fungi; Giant protoplast; Ultrastructure

【文獻(xiàn)出處】 食用菌學(xué)報(bào),Acta Edulis Fungi,編輯部郵箱,1996年02期 【DOI】CNKI:SUN:SYJB.0.1996-02-007