楊榮玲; 陳衛(wèi)東; 趙祥杰; 吳曉玉; 劉學(xué)銘; 徐玉娟
廣東省農(nóng)業(yè)科學(xué)院蠶業(yè)與農(nóng)產(chǎn)品加工研究所廣東省農(nóng)產(chǎn)品加工公共實(shí)驗(yàn)室; 廣東省農(nóng)業(yè)科學(xué)院蠶業(yè)與農(nóng)產(chǎn)品加工研究所廣東省農(nóng)產(chǎn)品加工公共實(shí)驗(yàn)室; 廣東省農(nóng)業(yè)科學(xué)院蠶業(yè)與農(nóng)產(chǎn)品加工研究所廣東省農(nóng)產(chǎn)品加工公共實(shí)驗(yàn)室; 江西農(nóng)業(yè)大學(xué)生物工程系; 廣東省農(nóng)業(yè)科學(xué)院蠶業(yè)與農(nóng)產(chǎn)品加工研究所廣東省農(nóng)產(chǎn)品加工公共實(shí)驗(yàn)室; 廣東省農(nóng)業(yè)科學(xué)院蠶業(yè)與農(nóng)產(chǎn)品加工研究所廣東省農(nóng)產(chǎn)品加工公共實(shí)驗(yàn)室 廣州510610; 江西農(nóng)業(yè)大學(xué)生物工程系; 江西南昌330045; 廣州510610; 廣州510610; 江西農(nóng)業(yè)大學(xué)生物工程系; 江西南昌330045; 江西南昌330045; 廣州510610; 廣州510610

【中文摘要】 以菌絲體生物量為指標(biāo),采用正交實(shí)驗(yàn)設(shè)計(jì)方法對(duì)巴西蟲(chóng)草液體培養(yǎng)的搖瓶生長(zhǎng)條件進(jìn)行了優(yōu)化研究。結(jié)果表明:優(yōu)化培養(yǎng)基的組成為可溶性淀粉2%、蛋白胨1%、大豆粉1%、KH2PO40.1%、MgSO4.7H2O 0.15%、VB10.01%、FeSO4.7H2O 0.002%、CaC l20.1%。該培養(yǎng)基下的菌絲體生物量為19.11g.L-1,比原培養(yǎng)基的發(fā)酵得率提高了26.01%;對(duì)巴西蟲(chóng)草液體培養(yǎng)的外部條件進(jìn)行優(yōu)化,確定搖瓶培養(yǎng)條件為:培養(yǎng)基初始pH 6.5、裝液量75mL/250mL、接種量7.5%、菌齡4d、發(fā)酵時(shí)間4d。研究了優(yōu)化后培養(yǎng)條件的菌體生長(zhǎng)代謝曲線,并得到了巴西蟲(chóng)草的液體培養(yǎng)條件和生長(zhǎng)動(dòng)力學(xué)曲線,為工業(yè)化生產(chǎn)提供了一定的理論依據(jù)。

【英文摘要】 The submerged culture conditions of Cordyceps brasiliensis Henn were optimized by orthogonal test.The results showed that the optimal medium ingredients were as follows: soluble starch 2%,peptone 1%,soybean 1%,KH_2PO_4 0.1%,MgSO_4·7H_2O 0.15%,VB_1 0.01%,FeSO_4·7H_2O 0.002%,and CaCl_2 0.1%.The mycelial biomass was 19.11 g·L-1 with this optimal medium to culture the fungus.The optimized external conditions were initial pH 6.5,medium capacity 75mL/250mL,seed age 4d,inoculum size 7.5% and culture time 4d.The growth curve of C. brasiliensis under the optimized condition was also investigated,and the mycelium yield was 19.11 g·L-1 which was 26.01% higher than with original medium.

【中文關(guān)鍵詞】 巴西蟲(chóng)草; 液體培養(yǎng); 優(yōu)化; 菌絲體生物量
【英文關(guān)鍵詞】 Cordyceps brasiliensis Henn.; Submerged culture; Optimization; Mycelial biomass
【基金】農(nóng)業(yè)部農(nóng)業(yè)結(jié)構(gòu)調(diào)整重大技術(shù)研究專(zhuān)項(xiàng)(06-08-03B);; 廣東省科技攻關(guān)項(xiàng)目(2005B20401020)

【文獻(xiàn)出處】 中國(guó)食用菌,Edible Fungi of China,編輯部郵箱,2007年01期 【DOI】CNKI:SUN:ZSYJ.0.2007-01-016