許旭萍; 李惠珍
福建師范大學(xué)生物系

【中文摘要】 本文采用一種較為簡單易行、高效、選擇性強的單孢分離法，分離獲得雙孢蘑菇的單孢菌林１５８株，并對這些單孢菌株的培養(yǎng)性狀、菌絲間融合和自發(fā)營養(yǎng)缺陷型等進行研究。結(jié)果表明：來自同一子實體的許多單孢子在ＰＤＡ培養(yǎng)基上其菌絲體形態(tài)、生長速度均有顯著差異：單孢菌株之間的融合率為１８．１％；單孢菌株中存在自發(fā)營養(yǎng)缺陷型，自然發(fā)生率為１０．１５％；活蘑菇菌絲體及高濃度孢子懸液對于單孢子萌發(fā)均具明顯的促進作用。

【英文摘要】 A simple,rapid and high selective method of monospore separation-is reported.158 monosporus strains of Agaricus bisporus were separated by this method.And thenthe studies on cultural harater,mycelial fusion and spontaneous auxotrophic mutant ofthese monosporous strains bad been done,The results show that monospones coming fromthe same fruitbody,when growing on PDA culture medium,show marked difference inmycelial morphologic character and growth speed;their mycelial fusion rate is 18.1%and the spontaneous auxotrophic mutant rate is 10.15%,Both of living mycelia ofAgaricus bisporus and high concentration of monosporus suspension are effectual tostimulate monospore to germinate.

【中文關(guān)鍵詞】 單孢分離; 單孢菌株; 菌絲融合; 自發(fā)營養(yǎng); 缺陷型; 萌發(fā)
【英文關(guān)鍵詞】 monospore separation; monosporus strain; mycelial fusion; spontaneous auxotrophic mutant; germinate

【文獻出處】 中國食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1995年05期 【DOI】CNKI:SUN:ZSYJ.0.1995-05-004