李成一; 王秉峰; 盧洪英; 路等學(xué)
甘肅省科學(xué)院生物研究所

【中文摘要】 平菇和香菇原生質(zhì)體融合育種研究簡(jiǎn)報(bào)李成一，王秉峰，盧洪英，路等學(xué)（甘肅省科學(xué)院生物研究所蘭州７３００００）平菇、香菇皆屬四極性異宗結(jié)合的食用菌類，其異核雙核體菌絲（簡(jiǎn)稱異核體）均具“鎖狀聯(lián)合（Ｃｌａｍｐｃｏｒｍｅｃｔｉｏｎ）”結(jié)構(gòu)。異核體菌絲經(jīng)酶解后...

【英文摘要】 Abstract The heterokaryonic mycelia of Pleurotns sapidus and Lentinus edodes had clampconnections(C.C.).Microscopic examinaatioti showed that after enzymolysis,theregenerative strains of protoplast possessed monokaryons or dikaryons.In this study, cell fusion was carried out using uninucleal protoplasts from P. sapldusand L. edodes, and using PEG-Ca as inducer. From 1022 regeneration strains,16fusion strains with C.C. were detected,Antagonistia experiment, di-mon mating,isoenzyrnepattern analysis, and harvesting experiment were conducted for 4 fusion strains showedthat. the fusions desinifely the character of the parents’,In addition,the fruit bodyyield of fusion strains all reached to commodity production level,and the yield of 3fusion strains was increased respectively by 1.6%,7.3%,and 11.4%as compared withthe parent P. sapldus.

【文獻(xiàn)出處】 中國(guó)食用菌,EDIBLE FUNGI OF CHINA,編輯部郵箱,1994年03期 【DOI】CNKI:SUN:ZSYJ.0.1994-03-007