野生硫磺菌分離株ITS序列分析
ITS Sequence Analysis of Strain ts Isolated from a Fruit Body of Laetiporus sulphureus Growing on Tai Mountain

【作者】 蘭玉菲； 王慶武； 安秀榮； 王明才；

【Author】 LAN Yufei,WANG Qingwu,AN Xiurong,WANG MingcaiEdible Fungi Research Institute,Tai’an Academy of Agricultural Sciences,Tai’an,Shandong 271000,China

【機(jī)構(gòu)】 泰安市農(nóng)業(yè)科學(xué)研究院食用菌研究所；

【摘要】 采用組織分離的方法從野生硫磺菌(Laetiporus sulphureus)子實(shí)體上分離得到菌株ts,以其基因組為模板擴(kuò)增獲得該菌株的ITS序列,序列分析結(jié)果表明:該菌株與GenBank中Laetiporus sulphureus var.sulphureus的3個(gè)日本分離株聚為一簇,ITS核苷酸一致率為98.31%～99.25%,而與其它22個(gè)來自不同國家的硫磺菌屬菌株的ITS核苷酸一致率為91.93%～98.12%,依此結(jié)果,ts菌株應(yīng)為L.sulphureus var.sulphureus。

【Abstract】 Fungal mycelium,isolated from a fruit body of Laetiporus sulphureus growing in the wild on a species of Quercus located on Tai Mountain,was identified as a strain(designated strain ts) of L.sulphureus var.sulphureus based on ITS sequencing.The ITS sequence(deposited in GenBank under the accession number,JF946748) was used along with ITS sequences from other Laetiporus species to construct a phylogenetic tree using the neighbor-joining method.This revealed a high genetic similarity(98.31%-99.25%)...

【關(guān)鍵詞】 硫磺菌硫磺原變種； 轉(zhuǎn)錄間隔區(qū)；
【Key words】 Laetiporus sulphureus var.sulphureus； internal transcribed spacers(ITS)；

【基金】 山東省中青年科學(xué)家科研獎(jiǎng)勵(lì)基金(編號(hào):BS2011NY021);泰安市科技發(fā)展計(jì)劃項(xiàng)目(編號(hào):20113008)的部分研究內(nèi)容

【分類號(hào)】S646;S567.39