野生赭鱗蘑菇及其分離菌株的鑒定Identification of an Agaricus subrufescens Strain from Zhejiang Province,China
【作者】 宋小亞; 劉德云; 李陽; 顧新偉; 吳春玲;
【Author】 SONG Xiaoya1,LIU Deyun1,LI Yang1,GU Xinwei2,WU Chunling1(1Edible Fungi Institute,Lishui Agricultural Academy of Sciences,Lishui,Zhejiang 323000,China;2Lishui Agricultural Bureau,Lishui,Zhejiang 323000,China)
【機構】 麗水市農(nóng)業(yè)科學研究院食用菌研究所; 麗水市農(nóng)業(yè)局;
【摘要】 依據(jù)形態(tài)學特征結合rDNA-ITS分析,對采集到的野生真菌進行分類鑒定;采用RAPD分析生物學手段快速判斷其分離菌株的真實性,并通過出菇試驗驗證判斷結果。該野生真菌鑒定為赭鱗蘑菇(Agaricus subrufescens),RAPD分析和出菇試驗結果表明分離菌株為該野生真菌的純培養(yǎng)物。
【Abstract】 Mushroom fruit bodies,collected from bamboo leaf piles near Bihu Town,Lishui City,Zhejiang Province,were identified as Agaricus subrufescens on the basis of sporophore and spore morphology,and ITS sequencing data.Pure cultures of fungal mycelium were obtained from fruit body tissue and confirmed as A.subrufescens on the basis of RAPD data and fruiting tests.
【關鍵詞】 野生真菌; 赭鱗蘑菇; 分離菌株; 鑒定;
【Key words】 Wild mushroom; Agaricus subrufescens; isolation of fungal mycelium; ITS sequencing;
【基金】 浙江省重大科技專項重點農(nóng)業(yè)項目(編號:2009C12096);浙江省食用菌產(chǎn)業(yè)創(chuàng)新團隊項目(編號:2011R09029-13)的部分研究內(nèi)容~~
【分類號】S646