草菇ras啟動(dòng)子區(qū)域的克隆及其序列分析

Cloning and Sequence Analysis of ras Promoter Region from Volvariella volvacea

劉世強(qiáng)　楊麗卿　郭麗瓊　林俊芳　

摘　要：根據(jù)Kajiwara S[1]等報(bào)道的ras啟動(dòng)子的序列設(shè)計(jì)并合成一對(duì)特異引物,以草菇菌絲的總DNA為模板,通過(guò)PCR擴(kuò)增,獲得一條長(zhǎng)約0.75kb片段.DNA序列測(cè)定結(jié)果表明,該片段長(zhǎng)度為751bp.采用Blast軟件和Promoter predictions軟件進(jìn)行啟動(dòng)子序列結(jié)構(gòu)分析,結(jié)果表明,該片段中243～293 bp和539～589 bp兩區(qū)域?yàn)閞as啟動(dòng)子的基礎(chǔ)啟動(dòng)子區(qū).在283bp和579bp處有兩個(gè)轉(zhuǎn)錄起始位點(diǎn),在244～247bp和292～295bp之間有2個(gè)TATA box,在36～39bp、377～380bp、434～437bp和716～719bp之間有4個(gè)CAAT box.此外,該片段中還含有9個(gè)G box、12個(gè)Ⅰ box、2個(gè)P box以及3個(gè)重要順式作用元件:ABRE元件、WUN-motif(基元)和HSE元件.

關(guān)鍵詞：草菇; ras啟動(dòng)子; PCR擴(kuò)增; 序列分析

分類(lèi)號(hào)：S646.130.6 　文獻(xiàn)標(biāo)識(shí)碼：A

文章編號(hào)：1005-9873(2004)01-0001-06

基金項(xiàng)目：國(guó)家自然科學(xué)基金資助項(xiàng)目(30060054和30371000)、廣東省自然科學(xué)基金資助項(xiàng)目(032239)和華南農(nóng)業(yè)大學(xué)校長(zhǎng)基金項(xiàng)目(5100-k03003)的部分研究?jī)?nèi)容

作者簡(jiǎn)介：劉世強(qiáng)(1978-),男,華南熱帶農(nóng)業(yè)大學(xué)農(nóng)學(xué)院在讀碩士研究生,主要從事食用菌的基因工程研究與開(kāi)發(fā).林俊芳,本文通訊作者

作者單位：劉世強(qiáng)（中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室,?？?571701）　

　　　　　楊麗卿（中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室,?？?571701）　

　　　　　郭麗瓊（中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室,?？?571701;華南農(nóng)業(yè)大學(xué)食品學(xué)院生物工程系,廣州,510640）　

　　　　　林俊芳（中國(guó)熱帶農(nóng)業(yè)科學(xué)院熱帶作物生物技術(shù)國(guó)家重點(diǎn)實(shí)驗(yàn)室,海口,571701;華南農(nóng)業(yè)大學(xué)食品學(xué)院生物工程系,廣州,510640）　

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