柱狀田頭菇生長(zhǎng)發(fā)育中9種胞外酶活性的測(cè)定

王南　沈鋒　譚琦　陳明杰　潘迎捷　

摘　要：柱狀田頭菇Agrocybeaegerita作為一種新開(kāi)發(fā)的食用菌，越來(lái)越受到消費(fèi)者的喜愛(ài)和研究者的關(guān)注，但在其栽培生產(chǎn)中遇到的產(chǎn)量相對(duì)較低的問(wèn)題，一直未能得到很好的解決，致使柱狀田頭菇的栽培難以得到大規(guī)模的推廣。本研究對(duì)不同來(lái)源的柱狀田頭菇在其6個(gè)不同生長(zhǎng)發(fā)育階段中，9種與培養(yǎng)料中主要組分分解相關(guān)的胞外酶活性的變化進(jìn)行了測(cè)定，結(jié)果表明：柱狀田頭菇屬褐腐菌，對(duì)非木質(zhì)纖維素的利用能力最強(qiáng)，對(duì)纖維素的利用能力較強(qiáng)，對(duì)木質(zhì)素的利用能力較差，但柱狀田頭菇具有漆酶活性。

關(guān)鍵詞：柱狀田頭菇，胞外酶，漆酶，培養(yǎng)料

分類號(hào)：Q936　文獻(xiàn)標(biāo)識(shí)碼：A

文章編號(hào)：1007-3515-(2000）04-0540-0546

DETECTING IN 9 EXTRACELLULAR ENZYME ACTIVITIES OF AGROCYBE AEGERITA STRAINS

WANG Nan（Key Lab. of Edible Fungi Genetics and Breeding， Agricultural Ministry of China Key Lab. of Agriculture Genetics and Breeding of Shanghai Edible Fungi Institute， Shanghai Academy of Agricultural Sciences Shanghai 201106）　

SHEN Feng（Key Lab. of Edible Fungi Genetics and Breeding， Agricultural Ministry of China Key Lab. of Agriculture Genetics and Breeding of Shanghai Edible Fungi Institute， Shanghai Academy of Agricultural Sciences Shanghai 201106）　

TAN Qi（Key Lab. of Edible Fungi Genetics and Breeding， Agricultural Ministry of China Key Lab. of Agriculture Genetics and Breeding of Shanghai Edible Fungi Institute， Shanghai Academy of Agricultural Sciences Shanghai 201106）　

CHEN Ming-Jie（Key Lab. of Edible Fungi Genetics and Breeding， Agricultural Ministry of China Key Lab. of Agriculture Genetics and Breeding of Shanghai Edible Fungi Institute， Shanghai Academy of Agricultural Sciences Shanghai 201106）　

PAN Ying-Jie（Key Lab. of Edible Fungi Genetics and Breeding， Agricultural Ministry of China Key Lab. of Agriculture Genetics and Breeding of Shanghai Edible Fungi Institute， Shanghai Academy of Agricultural Sciences Shanghai 201106）　

Abstract：As a kind of new edible mushroom breeds A. aegerita has been paid more attention with its unique flavor， abundant nutrition and medicinal value. But up to now it had not be solved that the yields of A. aegerita were lower than other cultivated mushroom. So that A. aegerita cultivation was difficult to popularize on a large scale. The activities of 9 extracellular enzymes were detected at 6 different stages of development for three different sources strains on the cultivated material. The results showed that A. aegerita belonged to brown rotten fungi. A. aegerita strains utilized the substrates of non-lignin-cellulose most strongly and degraded cellulose secondly. Lignin could not be utilized easily. But A. aegerita had laccase activity.

Keywords：A. aegerita， Extracellular enzyme， Laccase

作者單位：王南（上海市農(nóng)科院食用菌研究所農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)實(shí)驗(yàn)室上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室上海 201106）　

　　　　　沈鋒（上海市農(nóng)科院食用菌研究所農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)實(shí)驗(yàn)室上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室上海 201106）　

　　　　　譚琦（上海市農(nóng)科院食用菌研究所農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)實(shí)驗(yàn)室上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室上海 201106）　

　　　　　陳明杰（上海市農(nóng)科院食用菌研究所農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)實(shí)驗(yàn)室上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室上海 201106）　

　　　　　潘迎捷（上海市農(nóng)科院食用菌研究所農(nóng)業(yè)部食用菌遺傳育種重點(diǎn)實(shí)驗(yàn)室上海市農(nóng)業(yè)遺傳育種重點(diǎn)實(shí)驗(yàn)室上海 201106）　

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